OXYGEN CONSUMPTION

Seahorse XFe96

The Seahorse Bioscience XFe96 instrument, at Princess Margaret Cancer Research Tower in Toronto, measures the rate of change of analytes, dissolved oxygen and pH (lactate production) in the media immediately surrounding living cells cultured in a microplate (96-well). Changes in the extracellular media are caused by the consumption or production of analytes by the cells. Therefore, a sensitive measurement of the media flux can be used to determine rates of cellular metabolism with great precision and in a totally non-invasive, label-free manner. The instrument allows automated injection of up to 4 solutions at user defined time points during the experiment.

XFe96 Wave Desktop Software
XFe96 Wave Manual
Booking Calendar
Review Article: Assessing Mitochondrial Dysfunction
Advances in measuring cellular bioenergetics
Suspension Cells Protocol
Poly-D-Lysine Coating
Analyzing Isolated Mitochondria
Required Consumables FluxPack
Compounds Recipes
FAO Assay Setup
FAO Assay BSA-Palmitate conjugation
Cell Density and Oligomycin Optimization
FCCP Optimization with the XF Cell Mito Stress Test

Seahorse-xf96

To use the Seahorse Bioanalyzer XF 96 please, complete the form below to register for our next webinar training directed by Field Applications Scientists from Seahorse Bioscience.

Please, be advised that the instrument requires specialized consumables (see here). For ECAR measurements, a bicarbonate/buffer-free media is required and can be ordered from your media supplier prior to your first experiment.

Request Seahorse Training