The Seahorse Bioscience XF96 instrument, at Ontario Cancer Institute in Toronto, measures the rate of change of analytes, dissolved oxygen and pH (lactate production) in the media immediately surrounding living cells cultured in a microplate (96-well). Changes in the extracellular media are caused by the consumption or production of analytes by the cells. Therefore, a sensitive measurement of the media flux can be used to determine rates of cellular metabolism with great precision and in a totally non-invasive, label-free manner. The instrument allows automated injection of up to 4 solutions at user defined time points during the experiment.
Analyzing Isolated Mitochondria